RNA Preparation and Purification
TRIBIOSCIENCE INC Ribosome RNA Purification Kit, 20 Tests
Tribioscience’s Ribosome RNA Isolation Kit provides a streamlined approach to isolating ribosome RNA from different tissues and cells. Kit size is 20 sample preparation
Zymo Research Corporation RNA Clean & Concentrator™-100 (25 Preps) w/ Zymo-Spin™ V-E Columns w/ Reservoir (Capped)
This 15 minute procedure is based on the use of a unique single-buffer system and Zymo-Spin™ column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer to your sample, then bind, wash and elute ultra-pure RNA. The RNA can be eluted from the Zymo-Spin™ V-E Column in 100 l of RNase-free water. The highly concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Active Motif Low Cell ChIP Kit 16 rxns
Active Motif's Low Cell ChIP-Seq Kit provides a complete ChIP-Seq workflow for chromatin preparation, immunoprecipitation and NGS library preparation from limited amounts of cells or small tissue biopsies. The assay is robust enough to work with both high abundance histone modifications and low abundance transcription factor proteins. The Next Gen DNA Library and Next Gen DNA Indexing reagents are included so you can generate high complexity sequencing libraries that are compatible with Illumina platforms.
LGC GENOMICS LLC (Lucigen) MasterPure Yeast RNA Purification Kit, 100 PurificationsSafe, fast purification of high quality RNA from multiple species of yeast.
The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi.
Norgen Biotek Corp RNase-Free DNase I Kit, 200 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Research Products International Corp Direct-zol RNA MicroPrep w/Zymo-Spin IC Columns, Capped, 200 Preps
The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis.
Beckman Coulter RNAClean XP RNA and cDNA Cleanup Reagent, 450 mL (A66514)
Beckman Coulter RNAClean XP (A66514) is a paramagnetic bead-based reagent used to purify RNA and cDNA from common enzymatic reactions. Its SPRI (Solid Phase Reversible Immobilisation) chemistry binds nucleic acids for efficient cleanup and is compatible with manual and automated workflows.
- Magnetic bead-based reagent for RNA and cDNA cleanup.
- Uses SPRI paramagnetic bead chemistry to bind nucleic acids.
- Enables purification without centrifugation or filtration.
- Compatible with manual and automated workflows.
- Manufactured in an RNase-free environment.
- Shelf life of 18 months.
New England Biolabs, Inc. NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® – 96 reactions
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg.
- Generate the highest yields of high quality libraries, with a broad range of input amounts
- Increase the complexity and transcript coverage of your libraries
- Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
- Rely on robust performance, even with low quality RNA, including FFPE
New England Biolabs, Inc. NEBNext Ultra™ II RNA First Strand Synthesis Module – 96 reactions
This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina- compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1 g total RNA. The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming. The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module, and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1µg total RNA.
Zymo Research Corporation Quick-RNA™ MidiPrep Kit (25 Preps)
The Quick-RNA Midiprep Kit is designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.